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January 27, 2016
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Summary
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Zebrafish are an important model organism for the study of energy homeostasis. By utilizing a NADH2 sensitive redox indicator, alamar Blue, we have developed an assay that measures the metabolic rate of zebrafish larvae in a 96 well plate format and can be applied to drug or gene discovery.
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Williams, S. Y., Renquist, B. J. High Throughput Danio Rerio Energy Expenditure Assay. J. Vis. Exp. (107), e53297, doi:10.3791/53297 (2016).
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