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Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
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Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

Article DOI: 10.3791/53405 • 07:42 min • November 26th, 2015
November 26th, 2015 •
1, 1, 1, 1, 2, 1
1Department of Pathology, University of Illinois at Chicago, 2Department of Periodontics, University of Illinois at Chicago

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Summary
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The goal of this protocol is to use laser-capture micro-dissection as an effective method to isolate pure populations of cell types from heterogeneous prostate tissues for downstream RNA analysis.

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Laser-capture Microdissection Human Prostatic Epithelium RNA Analysis Prostate Gland Stromal Cell Types Epithelial Cell Types Neuroendocrine Cells Immune Cells Prostate Cancer Dysplastic Luminal Epithelium Stromal Population Gene Expression Studies Benign Prostate Laser-capture Microdissection (LCM) RNA Isolation Gene Expression Analysis RT-qPCR RNAseq
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