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JoVE Journal
Neuroscience

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Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains
 

Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains

Article DOI: 10.3791/53415
January 5th, 2016

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Summary January 5th, 2016

Here, we present a protocol for the isolation of increasingly insoluble/aggregated alpha-synuclein (α-syn) from post-mortem human brain tissue. Through the utilization of buffers with increasing detergent strength and high-speed ultracentrifugation techniques, the variable properties of α-syn aggregation in diseased and non-diseased tissue can be examined.

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