Cetacean Myoglobin Detection
Cetacean Myoglobin Detection - Related Videos
11:48
Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin
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Cited by 5 •
2016
This protocol describes the development of two IgG class monoclonal antibodies (mAbs) strongly reactive to myoglobin of cetaceans. These mAbs are applied on a colloidal gold immunochromatographic test strip based on the sandwich format to differentiate the Mb of cetaceans from seal and other animals.
07:05
Use of Autometallography to Localize and Semi-Quantify Silver in Cetacean Tissues
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Cited by 2 •
2018
A protocol is presented to localize Ag in cetacean liver and kidney tissues by autometallography. Furthermore, a new assay, named the cetacean histological Ag assay (CHAA) is developed to estimate the Ag concentrations in those tissues.
08:51
The Use of Thermal Infra-Red Imaging to Detect Delayed Onset Muscle Soreness
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Cited by 110 •
2012
The purpose of this investigation was to assess whether using an infra-red thermal camera is a valid tool for detecting and quantifying the muscle soreness after exercising.
12:32
Image Rendering Techniques in Postmortem Computed Tomography: Evaluation of Biological Health and Profile in Stranded Cetaceans
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Cited by 13 •
2020
The Hong Kong cetacean stranding response program has incorporated postmortem computed tomography, which provides valuable information on the biological health and profile of the deceased animals. This study describes 8 image rendering techniques that are essential for the identification and visualization of postmortem findings in stranded cetaceans, which will help clinicians, veterinarians and stranding response personnel worldwide to fully utilize the radiological modality.
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
FAQs
The heme group gives myoglobin a characteristic analytical signal that can be measured in a tissue or biological sample. Detecting this signal indicates the presence of the oxygen-binding protein and can support estimates of its abundance. Because heme is central to myoglobin’s oxygen-related function, signal-based measurements connect molecular detection with muscle oxygen availability during diving.
Antibodies provide a specificity-based approach by binding to myoglobin rather than relying only on its heme-related signal. This selective recognition helps distinguish the target protein within a complex biological sample. Antibody-based detection can therefore complement signal measurement when researchers need evidence that the detected material is specifically myoglobin.
Differences in myoglobin abundance can be examined alongside oxygen capacity, locomotion, depth, and dive duration. Greater or lower amounts may help researchers characterize how muscle supports prolonged submergence, while comparisons among whales and dolphins can reveal variation in diving-related physiology. These measurements also contribute to studies of cetacean evolutionary history.
Species-level differences provide comparative evidence about muscle oxygen capacity and possible associations with diving behavior, but the measurements should be considered in relation to depth, dive duration, and locomotion. A comparison can identify patterns of physiological adaptation without treating myoglobin abundance alone as a complete explanation for every species’ diving performance.
A study begins with a whale or dolphin tissue or other biological sample, followed by analysis for myoglobin using either its characteristic heme-related signal or an antibody that binds the protein. Researchers then compare the resulting measurements across samples, individuals, or species. The workflow links molecular detection to questions about muscle oxygen availability and diving physiology.
The measurements can support comparative studies of whale and dolphin muscle, including investigations of oxygen capacity, locomotion, depth adaptation, and dive duration. They also provide data for examining relationships between protein abundance and cetacean evolutionary history. In biology, the approach connects a measurable molecular feature with broader patterns of physiology and adaptation.