Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
 

Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization

Article DOI: 10.3791/53445
December 23rd, 2015

Chapters

Summary December 23rd, 2015

This is a quick, cost-efficient protocol for the production of secreted, glycosylated mammalian proteins and subsequent single-step purification with sufficient yields of homogenous protein for X-ray crystallography and other biophysical studies.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter