A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

The overall goal of this method is to prepare plant chromosomes suitable for the fluorescence in C two hybridization technique using single and high copy DNA probes. This method can help answer key questions in cytogenetics and genome research, such as detection of chromosome rearrangements, ordering of genetic markers on chromosomes, and comparative analysis between different species. The main advantage of this procedure is that preparation of chromosome spreads becomes fast, easy, and efficient.

Cell suspension can be stored at minus 20 degree up to two months and used for chromosome preparation when needed. We first had this idea of this method when we observed that the quality and the number of suitable chromosomes increased dramatically are human days Germinate 10 to 20 barley seeds on two layers of moist filter paper in a Petri dish under dark conditions for two days at 22 to 24 degrees Celsius after two days. Cut off vigorous roots with lengths of one to two centimeters from the seed by using a razor blade.