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A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants

Article DOI: 10.3791/53470-v • 09:08 min • December 16th, 2015
December 16th, 2015 •
1, 1,2, 1
1Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 2School of Biological and Chemical Sciences, Queen Mary University of London

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Summary
Automatic Translation

A protocol is described for the preparation of high-quality mitotic plant chromosome spreads by a fast air-dry dropping method suitable for the FISH detection of single and high copy DNA probes.

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Chromosome Preparation Method FISH In Plants Drop Preparation Air-drying Technique Mitotic Chromosome Spreads DNA Probes Relative Humidity Washing Steps Efficient And Reproducible Metaphase Chromosomes Hordeum Vulgare H. Bulbosum H. Marinum H. Murinum H. Pubiflorum Secale Cereale
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