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JoVE Journal
Immunology and Infection

This content is Open Access.

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
 

A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood

Article DOI: 10.3791/53485-v 08:17 min February 8th, 2016
February 8th, 2016

Chapters

Summary

Whole blood Immunophenotyping is indispensable for monitoring the human immune system. However, the number of reagents required and the instability of specific fluorochromes in premixed cocktails necessitates daily reagent preparation. Here we show that semi-automated preparation of staining antibody cocktail helps establish reliable immunophenotyping by minimizing variability in reagent dispensing.

Tags

Semi-automated Approach Antibody Cocktails Immunophenotypic Analysis Human Peripheral Blood Reagent Dispensing Immunomonitoring Pipetting Accuracy Manual Cocktail Preparation Technician 2D Barcode Tubes Antibody Transfer Barcode Reader Spreadsheet Comma Separated Values File Automated Liquid Handler Syringes
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