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人生长激素测定血清SPRI,纳米单层屋面和酶联免疫吸附测定比较分析
JoVE Journal
Biology
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JoVE Journal
Biology
Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
Please note that all translations are automatically generated.
Click here for the English version.
人生长激素测定血清SPRI,纳米单层屋面和酶联免疫吸附测定比较分析
DOI:
10.3791/53508-v
•
11:17 min
•
January 07, 2016
•
Stephen Vance*
1
,
Effat Zeidan*
1
,
Vincent C. Henrich
,
Marinella G. Sandros
1
Department of Nanoscience
,
University of North Carolina at Greensboro
,
2
Center for Biotechnology, Genomics, and Health Research
,
University of North Carolina at Greensboro
,
3
HORIBA Scientific
Chapters
00:05
Title
01:15
Prepare SPRi Chip for Antibody Array
04:08
SPRi Detection of Human Growth Hormone
05:46
ELISA Protocol
08:21
Results: SPRi, Nano-SPRi, and ELISA Analysis of Recombinant Human Growth Hormone
09:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
所提出的工作(SPRI)测定法,特别是用于在加标人血清重组人生长激素的检测,通过比较SPRI直接结果与市售的酶联免疫吸附试验评估直接和扩增表面等离子体共振成像的诊断潜力(ELISA)套件。
Tags
SPRi
Nano-SPRi
ELISA
HGH
Serum
Biomarker Detection
Label-free
Real-time Monitoring
Biochip Surface Chemistry
11-mercaptoundecanoic Acid
1-Ethyl-3-carbodiimide
N-Hydroxysuccinimide
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