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药物与DNA相互作用的细胞全基因组映射与COSMIC(小分子交联,染色质隔离)
JoVE Journal
Biology
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JoVE Journal
Biology
Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)
Please note that all translations are automatically generated.
Click here for the English version.
药物与DNA相互作用的细胞全基因组映射与COSMIC(小分子交联,染色质隔离)
DOI:
10.3791/53510-v
•
10:05 min
•
January 20, 2016
•
Graham S. Erwin
,
Matthew P. Grieshop
,
Devesh Bhimsaria
2
,
Asuka Eguchi
,
José A. Rodríguez-Martínez
,
Aseem Z. Ansari
4
1
Department of Biochemistry
,
University of Wisconsin–Madison
,
2
Department of Electrical and Computer Engineering
,
University of Wisconsin–Madison
,
3
Graduate Program in Cellular and Molecular Biology
,
University of Wisconsin–Madison
,
4
The Genome Center
,
University of Wisconsin–Madison
Chapters
00:05
Title
00:48
Crosslinking in Live Cells
03:19
Isolation of Chromatin
05:14
Capture of Ligand-DNA Crosslinks and Isolation of Affinity-purified DNA
07:52
Results: COSMIC Indentifies Genome-wide Binding Events of Polyamides
09:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
识别基因组靶向分子的直接目标仍然是一个重大的挑战。要了解DNA结合分子如何参与基因组,我们开发了依靠小分子交联,染色质隔离的方法(COSMIC)。
Tags
Genome-wide Mapping
Drug-DNA Interactions
COSMIC
Crosslinking
Small Molecules
Chromatin
Polyamides
DNA-targeting
Mechanism Of Action
Therapeutic Agents
DNA-targeting Molecules
Pluripotent Stem Cells
Photo-cross-linking
UV Radiation
Cell Dissociation
Enzyme
E8 Media
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