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A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response
 

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

Article DOI: 10.3791/53513
April 17th, 2016

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Summary April 17th, 2016

This article describes the methods for screening the genes controlling plasmodesmal permeability and hence auxin gradient during tropic response. This includes the measurement of the degree of tropic response in hypocotyl of Arabidopsis thaliana and checking plasmodesmal permeability by 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) loading and finally callose level assessment.

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