Simultaneous Two-photon <em>In Vivo </em>Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Kacper &#321;ukasiewicz; Magdalena Robacha; &#321;ukasz Bo&#380;ycki; Kasia Radwanska; Rafa&#322; Czajkowski

doi:10.3791/53528

Journal

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Neuroscience

/

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Simultaneous Two-photon <em>In Vivo </em>Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

JoVE Journal
Neuroscience
Author Produced

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JoVE Journal Neuroscience

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

11,470 Views

•

16:45 min

•

March 13, 2016

DOI:

10.3791/53528-v

DOI:

10.3791/53528-v

•

16:45 min

March 13, 2016

•

11439 Views

Kacper Łukasiewicz*¹, Magdalena Robacha*¹, Łukasz Bożycki², Kasia Radwanska¹, Rafał Czajkowski³

¹Department of Molecular and Cellular Neuroscience,Nencki Institute of Experimental Biology ²Department of Biochemistry,Nencki Institute of Experimental Biology ³Neurobiology Centre,Nencki Institute of Experimental Biology

Summary

This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC.