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Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
Simultaneous Two-photon <em>In Vivo </em>Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

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16:45 min

March 13, 2016

DOI:

16:45 min
March 13, 2016

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Summary

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This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC.

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