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Biology

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High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
 

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

Article doi: 10.3791/53568
December 28th, 2015

Summary December 28th, 2015

Laboratory-scale production of eukaryotic proteins with appropriate post-translational modification represents a significant barrier. Here is a robust protocol with rapid establishment and turnaround for protein expression using a mammalian expression system. This system supports selective amino acid, selective labeling of proteins and small molecule modulators of glycan composition.

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