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Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo
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JoVE Journal Neuroscience
Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo

Induction of an Isoelectric Brain State to Investigate the Impact of Endogenous Synaptic Activity on Neuronal Excitability In Vivo

DOI:
10.3791/53576-v

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10:19 min

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March 31, 2016

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, , , ,
1Inserm U1127, 2CNRS UMR 7225, 3UPMC Univ Paris 06, UMR S 1127,Sorbonne Universités, 4Institut du Cerveau et de la Moelle épinière (ICM)

Chapters

  • 00:05Title
  • 00:47Surgical Preparation
  • 05:21Intracellular Recordings
  • 06:54Induce the Isoelectric State
  • 08:27Results: Consequences of Synaptic Activity Suppression on the Membrane Potential Dynamics and on Cortical Neurons Excitability
  • 09:44Conclusion

Summary

Automatic Translation

Automatic Translation

This procedure performs long-lasting in vivo intracellular recordings from single neurons during physiologically relevant cerebral states and after complete abolition of ongoing electrical activities, resulting in an isoelectric brain state. The physiological constants of the animal are carefully monitored during the transition to the artificial comatose condition.

Tags

Keywords: Isoelectric Brain StateNeuronal ExcitabilityIn VivoSynaptic ActivityAnesthesiaTracheal IntubationPeritoneal CatheterPhysiological MonitoringStereotaxic Frame

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