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Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding
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Bioengineering
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JoVE Journal Bioengineering
Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

DOI:
10.3791/53642-v

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09:14 min

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August 22, 2016

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, , ,
1Department of Biochemistry, Yong Loo Lin School of Medicine,National University of Singapore, 2Epithelial Biology Laboratory,Institute of Medical Biology, A*STAR, 3Department of Biomedical Engineering, Faculty of Engineering,National University of Singapore

Chapters

  • 00:05Title
  • 01:16Macromolecular Crowding in 2D Skin Cell Cultures
  • 02:06Making and Using a Cell-derived Matrix
  • 03:393D Organotypic Skin Co-culture
  • 06:24Results: Macromolecular Crowding of Fibroblasts and Keratinocytes
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol to obtain cell-derived matrices rich in extracellular matrix proteins, using macromolecular crowders (MMC). In addition, we present a protocol which incorporates MMC in 3D organotypic skin co-culture generation, which reduces culture time while maintaining maturity of construct.

Tags

Macromolecular CrowdingExtracellular Matrix Deposition2D And 3D Skin ModelsCell-derived MatricesOrganotypic Skin ConstructsWound TherapiesSkin EquivalentsPrimary FibroblastsPrimary KeratinocytesCo-cultureDecellularizationSodium DeoxycholateCell-derived Matrix

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