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JoVE Journal
Bioengineering

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Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding
 

Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding

Article doi: 10.3791/53642
August 22nd, 2016

Summary August 22nd, 2016

We present a protocol to obtain cell-derived matrices rich in extracellular matrix proteins, using macromolecular crowders (MMC). In addition, we present a protocol which incorporates MMC in 3D organotypic skin co-culture generation, which reduces culture time while maintaining maturity of construct.

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