3d Skin Model
3d Skin Model - Related Videos
04:07
Generation of Organotypic Full Skin Reconstructs: A Procedure to Establish 3D Skin Model Using Human Skin Samples
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2023
This video describes the detailed protocol for generating in vitro 3D organotypic skin reconstructs to study melanoma progression. This skin model mimics both the architecture and multicellular complexity of organs in vivo.
09:14
Improving 2D and 3D Skin In Vitro Models Using Macromolecular Crowding
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Cited by 14 •
2016
We present a protocol to obtain cell-derived matrices rich in extracellular matrix proteins, using macromolecular crowders (MMC). In addition, we present a protocol which incorporates MMC in 3D organotypic skin co-culture generation, which reduces culture time while maintaining maturity of construct.
08:49
A 3D Organotypic Melanoma Spheroid Skin Model
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Cited by 50 •
2018
Here, we present a protocol to generate a 3D organotypic melanoma spheroid skin model that recapitulates both the architecture and multicellular complexity of an organ/tumor in vivo but at the same time accommodates systematic experimental intervention.
03:02
Chemical-induced Two-stage Skin Carcinogenesis Model: An Experimental In Vivo Mouse Model of Skin Cancer
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2023
This video describes the procedure to induce two-stage skin carcinogenesis to study tumor initiation by topical application of chemicals. In the first step, a mutagen, DMBA, is applied onto the skin of a mouse model which initiates tumor formation by causing mutations in the stem cells, followed by the application of a growth stimulator, TPA, which accelerates skin papilloma formation.
11:02
The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal Skin and Melanoma Progression
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Cited by 111 •
2011
In this report, we describe the three-dimensional skin reconstruct model which mimics human skin in architecture and composition. Melanocyte physiology, melanoma progression and the fate of dermal stem cells have been investigated using the skin reconstruct model. The model is also useful as a preclinical tool for drug assessment.
FAQs
Air-liquid interface culture promotes epidermal stratification and barrier formation, adding organization to the engineered tissue. The resulting model better reflects key features of skin structure and function, allowing investigators to study cancer processes in a more tissue-relevant setting. This is particularly useful when tumor behavior depends on tissue organization or interactions with surrounding cells.
Their three-dimensional architecture provides a tissue context in which cancer cells can be examined alongside skin cells and an extracellular-matrix scaffold. Investigators can therefore study tumor initiation and invasion as tissue-level processes, while also examining interactions between cancer cells and their surrounding environment. This expands analysis beyond cancer-cell behavior in isolation.
Instead of evaluating cancer cells as an isolated population, the model incorporates skin-related cellular and structural features, including keratinocytes, fibroblasts, optional melanocytes, and a collagen or extracellular-matrix scaffold. Researchers can examine tumor behavior within a reconstructed tissue environment and assess responses in relation to skin structure, rather than measuring cancer-cell effects alone.
Researchers assemble the model from relevant skin cells within a collagen or extracellular-matrix scaffold, then culture the construct under conditions that allow epidermal stratification and barrier formation. Cancer cells can subsequently be studied in this reconstructed tissue context, supporting experiments on tumor initiation, invasion, cell-tissue interactions, or treatment response.
These models support testing of anticancer drugs and topical treatments, as well as observation of therapeutic responses in a skin-relevant setting. Their tissue organization allows investigators to examine treatment effects alongside tumor behavior and the surrounding reconstructed tissue. The same platform can connect drug evaluation with studies of tumor initiation and invasion.
Results can show how cancer cells initiate tumors, invade reconstructed tissue, interact with surrounding cells and matrix, or respond to an intervention. Because the system reproduces key structural and functional features of skin, observations can be interpreted in relation to tissue context. This helps link anticancer activity with changes in tumor behavior.
They provide a human skin-based laboratory system for investigating cancer-related processes and evaluating treatments, including topical approaches. By reproducing important aspects of skin structure and function in culture, they can support experiments that might otherwise rely on animal models. The source material identifies reduced reliance on animal models as a major research advantage.