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JoVE Journal
Developmental Biology

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Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos
 

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Article doi: 10.3791/53654
February 22nd, 2016 Usage Statistics

Summary February 22nd, 2016

This protocol presents a method to perform quantitative, single-cell in situ analysis of protein expression to study lineage specification in mouse preimplantation embryos. The procedures necessary for collection of blastocysts, whole-mount immunofluorescent detection of proteins, imaging of samples on a confocal microscope, and nuclear segmentation and image analysis are described.

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