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A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells

A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells

DOI:
10.3791/53655-v

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08:34 min

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March 03, 2016

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, ,
1Institute of Clinical Biochemistry,Hannover Medical School

Chapters

  • 00:05Title
  • 00:34Human ES Cell Differentiation toward the Definitive Endoderm
  • 02:57CXCR4+ Definitive Endoderm (DE) Cell Immunostaining for Flow Cytometric Analysis
  • 04:55CXCR4+ Cell Magnetic Bead Isolation
  • 06:34Results: Representative Changes in Gene/Protein Expression after Human ESC into DE Differentiation
  • 07:59Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a method for the purification of differentiated human embryonic stem cells that are committed towards the definitive endoderm for the improvement of downstream applications and further differentiations.

Tags

PurificationEndoderm CellsHuman Embryonic Stem CellsTechniqueUndifferentiated CellsCell Culture PlatesBasement Membrane MatrixHarvestPBSEnzyme-free Passaging Solution ReagentDMMF12 MediumCentrifugation Tube

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