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Journal
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Immunology and Infection
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分离和胶质瘤浸润外周血单个核细胞的流式细胞仪分析
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
Please note that all translations are automatically generated.
Click here for the English version.
分离和胶质瘤浸润外周血单个核细胞的流式细胞仪分析
DOI:
10.3791/53676-v
•
12:52 min
•
November 28, 2015
•
Gregory J. Baker
2
,
Maria G. Castro
2
,
Pedro R. Lowenstein
2
1
Department of Neurosurgery
,
University of Michigan
,
2
Department of Cell and Developmental Biology
,
University of Michigan
Chapters
00:05
Title
00:46
Stereotactic Engraftment of Glioma Cells into Mouse Striatum
02:52
Mouse Transcardial Perfusion and Brain Harvesting
04:31
Isolation of Glioma-infiltrating PBMCs
09:40
Results: Flow Cytometric Gating Strategy Used to Identify Glioma-infiltrating Gr-1+/ CD11b+ Myeloid Cells and NK.1 + Cells
11:27
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是胶质瘤浸润外周血单核细胞的分离和流式细胞术分析能够产生在进入早期脑肿瘤微环境的免疫细胞的数目和激活状态依赖于时间的定量数据一个直接的方法。
Tags
Glioma
Peripheral Blood Mononuclear Cells
Flow Cytometry
Natural Killer Cells
Myeloid Cells
Galectin-1
Tumor Microenvironment
Immune Infiltration
Brain Tumor
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