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Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
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Immunology and Infection
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JoVE Journal Immunology and Infection
Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

DOI:
10.3791/53682-v

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08:34 min

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March 02, 2016

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,
1Department of Medicine, Division of Immunobiology,University of Vermont, 2Department of Microbiology & Molecular Genetics,University of Vermont

Chapters

  • 00:05Title
  • 00:27Prepare Medium, Seed Cells in 48-well Plates, and Carry Out Virus-cell Attachment
  • 02:52Viral RNA Extraction
  • 04:22qRT-PCR Measurement of Viral Genome
  • 06:38Results: Arenavirus Cell Attachment Measurements
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

The first step in the arenavirus life cycle is attachment of viral particles to host cells. We report a quantitative (q)RT-PCR-based assay for ultrasensitive detection and quantitation of arenavirus attachment events.

Tags

Highly Sensitive AssayMeasurementArenavirus-cell AttachmentTechniqueSensitivityAdaptationVirion EndocytosisGenome ReleaseDMEMHeat Inactivated Fetal Bovine SerumPenicillin-streptomycinHEPES BufferVero E6 CellsIncubationJunin Candid 1 Virus SamplesFocus Forming Units (FFUs)Plaque Forming Units (PFUs)Inoculation

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