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Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
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Developmental Biology
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JoVE Journal Developmental Biology
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

DOI:
10.3791/53696-v

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08:43 min

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June 06, 2016

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, , , , ,
1Department of Nanobiomedical Sciences and BK21 PLUS NBM Global Research Center for Regenerative Medicine,Dankook University, 2Insititute of Tissue Regeneration Engineering,Dankook University, 3Department of Biological Sciences,Ajou University, 4Department of Pharmacy,Sahmyook University, 5Department of Animal Biotechnology,Sahmyook University

Chapters

  • 00:05Title
  • 01:13Oviduct Isolation
  • 02:17Retrieval and Culturing of 2-Pronuclear (2-PN) Embryos
  • 03:46Microinjecting 2-PN Embryos
  • 06:29Results: Neogenin Expression Manipulations
  • 07:59Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to describe a loss- and gain-of function method that is applicable to identify neogenin as a stage-specific receptor that leads to trophectoderm and inner cell mass differentiation in preimplantation mouse embryos.

Tags

Loss-of-functionGain-of-functionEarly Cell Fate DeterminantsPreimplantation Mouse EmbryosInner Cell Mass DifferentiationStage-specific MoleculesDevelopmental BiologyStem Cell BiologyAnimal ScienceReproductive BiotechnologyAnimal CloningReceptor-ligand InteractionEmbryonic Stem Cell-specific FactorCollaboration With Multiple LaboratoriesProfessor Sang Jin LeeYong Il ChoInjectionSacrifice Pregnant FemalesCarbon Dioxide IntoxicationCervical DislocationUterine HornsBody Cavity

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