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JoVE Journal Neuroscience
In Vivo Functional Brain Imaging strategi som bygger på Bioluminescent Kalcium indikator GFP-aequorin
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In Vivo Functional Brain Imaging strategi som bygger på Bioluminescent Kalcium indikator GFP-aequorin

Article DOI: 10.3791/53705-v • 12:15 min • January 8th, 2016
January 8th, 2016 •
1,2, 2,3, 1
1Equipe: Imagerie Cérébrale Fonctionnelle et Comportements (ICFC), Institut des Neurosciences Paris-Saclay (Nero-PSI), UMR-9197, CNRS/Université Paris Sud, 2Interdisciplinary Program in Neuroscience, Graduate College, University of Iowa, 3Department of Anesthesia, Carver College of Medicine, University of Iowa

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Här presenterar vi en ny Ca2 + -imaging tillvägagångssätt med hjälp av en självlysande reporter. Detta tillvägagångssätt använder en kondenserad konstrukt GFP-aequorin som binder till Ca 2+ och emitterar ljus, vilket eliminerar behovet av ljusexcitering. Betydligt denna metod medger lång kontinuerlig avbildning, tillgång till djupa hjärnstrukturer och hög tidsupplösning.

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Neuroscience Utgåva 107 Ca självlysande reporter GFP-Aequorin svamp kropp P2X Drosophila spontan aktivitet
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