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RNAi Trigger Delivery into Anopheles gambiae Pupae
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RNAi Trigger Delivery into Anopheles gambiae Pupae

RNAi Trigger Delivery into Anopheles gambiae Pupae

DOI:
10.3791/53738-v

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09:58 min

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March 08, 2016

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, , , , ,
1Biology Department,Boston College, 2Division of Biology,Kansas State University, 3Discovery Research,Biogen

Chapters

  • 00:05Title
  • 00:57Synthesis and Preparation of Double Stranded RNA (dsRNA)
  • 02:10Preparations for dsRNA Injections
  • 04:07Microinjecting Pupae with dsRNA
  • 06:22Culturing and Assessing Injected Pupae
  • 07:12Results: Injection Survivorship and SRPN2 Knockdown by dsRNA Injection
  • 08:34Conclusion

Summary

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RNA interference (RNAi) is an extremely valuable tool for uncovering gene function. However, the ability to target genes using RNAi during pre-adult stages is limited in the major human malaria vector Anopheles gambiae. We describe an RNAi protocol to reduce gene function via direct injection during pupal development.

Tags

RNAi Trigger DeliveryAnopheles GambiaeGene KnockdownPupal StageVector BiologyFunctional Genomic StudiesRNA Interference Injection ProtocolsPrecision InjectionDouble-stranded RNA SynthesisT7 Promoter SequenceIn Vitro KitRNA ConcentrationAgarose GelDouble-stranded RNA Band

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