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Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques
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Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques

Article DOI: 10.3791/53748-v • 11:49 min • March 24th, 2016
March 24th, 2016 •
1, 1, 1
1ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, University of Melbourne

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This protocol describes the specific techniques used for the structural characterization of reducing end (RE) and internal region glycosyl sequence(s) of heteroxylans by tagging the RE with 2 aminobenzamide prior to enzymatic (endoxylanase) hydrolysis and then analysis of the resultant oligosaccharides using mass spectrometry (MS) and nuclear magnetic resonance (NMR).

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Sequencing Plant Wall Heteroxylans Enzymic Techniques Chemical Techniques Methylation Physical Techniques Mass Spectrometry Nuclear Magnetic Resonance Characterize Reducing End And Internal Region Glycosal Sequences Water-soluble Heravenaxylans Endoxylanase Label Dolara Saccharides Glycomic Field Fine Structure Alterations Of Oligosaccharides Or Polysaccharides Growth Development Abiotic Stressors Biotic Stressors Integrated Approach To Sequencing One Molar Sodium Cyanoborohydride Solution 2AB Reagent PH 5.5 Acetic Acid Water-soluble Arabinoxylans Vortex Mixer Incubation At 65 Degrees Celcius Fume Hood
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