Sequencing van Plant Muur Heteroxylans Met behulp van Enzymatische, Chemical (Methylering) en fysieke (Mass Spectrometry, Nuclear Magnetic Resonance) Technieken
This protocol describes the specific techniques used for the structural characterization of reducing end (RE) and internal region glycosyl sequence(s) of heteroxylans by tagging the RE with 2 aminobenzamide prior to enzymatic (endoxylanase) hydrolysis and then analysis of the resultant oligosaccharides using mass spectrometry (MS) and nuclear magnetic resonance (NMR).