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Analyse von LINE-1-Retrotransposition im Einzel Nucleus Ebene
JoVE Journal
Biology
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JoVE Journal Biology
Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
DOI:

11:52 min

April 23, 2016

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Chapters

  • 00:05Title
  • 00:44Chemical Labeling
  • 02:18Preparing Chromosome Spreads
  • 05:28Stabilizing, Dehydrating and Hybridization of Spreads with L1-labeled Retrotransposition Probes
  • 07:26Washing or Addition of Secondary Antibody and Viewing
  • 08:49Results: Long Interspersed Nuclear Element-1, or LINE-1 Retrotransposition Analysis
  • 10:38Conclusion

Summary

Automatic Translation

Hier beschäftigen wir FISH - Methodik LINE-1 - Retrotransposition an der einzelnen Kerne Ebene in Chromosom Spreads von HepG2 - Zelllinien stabil synthetischen LINE-1 exprimieren , zu verfolgen.

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