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Biology

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Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
 

Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs

Article DOI: 10.3791/53782
June 17th, 2016

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Summary June 17th, 2016

This protocol describes an isolation technique for obtaining primary lung resident mesenchymal stromal cells from rats, through the use of enzymatic digestion, density gradient separation, plastic adherence and CD146+ magnetic bead selection.

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