Msc Surface Markers
Msc Surface Markers - Related Videos
11:13
MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells
0 Views •
Cited by 47 •
2017
Here, a protocol (MitoCeption) is presented to transfer mitochondria, isolated from human mesenchymal stem cells (MSC), to glioblastoma stem cells (GSC), with the goal of studying their biological effects on GSC metabolism and functions. A similar protocol can be adapted to transfer mitochondria between other cell types.
08:52
Isolation and Enrichment of Liver Progenitor Subsets Identified by a Novel Surface Marker Combination
0 Views •
Cited by 3 •
2017
Liver injuries are accompanied by progenitor cell expansion that represents a heterogeneous cell population. Novel classification of this cellular compartment allows for the distinguishing of multiple subsets. The method described here illustrates the flow cytometry analysis and high purity isolation of various subsets that can be used for further assays.
03:20
Labeling Neural Cell Surface Markers with Azidosugar while Co-culturing with Endothelial Cells
0 Views •
2025
This video demonstrates a protocol for co-culturing mouse brain endothelial cells and primary cortical stem cells and labeling the cell surface glycoproteins using Ac4ManNAz (azidosugar per-O-acetylated N-azidoacetylmannosamine). This method allows for selective enrichment and identification of cell surface proteins in primary cells.
10:32
Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
0 Views •
Cited by 21 •
2014
Mouse embryonic fibroblast can be reprogrammed into induced pluripotent stem cells at low efficiency by the forced expression of transcription factors Oct-4, Sox-2, Klf-4, c-Myc. The rare intermediates of the reprogramming reaction are FACS isolated via labeling with antibodies against cell surface makers Thy-1.2, Ssea-1, and Epcam.
04:49
MSC Seeded Scaffold Preparation: A Technique to Prepare Scaffold Seeded with Mesenchymal Stem Cells for Surgical Implantation into Murine Model
0 Views •
2025
This video describes the technique of seeding mesenchymal stem cells (MSCs) onto a biodegradable poly(lactic acid) (PLA) scaffold. The prepared scaffolds can be implanted into the surgical resection cavity of the brain from where they may migrate towards tumor foci. Further, these cells can be engineered to express therapeutic proteins to stimulate apoptosis in cancer cells.
FAQs
Positive markers such as CD73, CD90, and CD105 provide evidence that a cultured cell population has the expected MSC-associated surface profile. Their value increases when the profile is considered as a group rather than as a single measurement. This multi-marker view helps characterize cultures and supports comparisons between samples without treating one protein as definitive.
Negative markers serve a complementary purpose: they help exclude cells associated with hematopoietic or endothelial populations. This is important because detecting positive MSC-associated markers alone does not establish that unwanted cell types are absent. Including both positive and negative assessments therefore gives a more informative description of culture composition and strengthens marker-based quality control.
Fluorescently labeled antibodies provide the detection mechanism by binding selected proteins on the cell membrane. The resulting fluorescence identifies cells carrying the corresponding marker and can be examined by flow cytometry or immunostaining. Because the readout depends on antibody binding to specific membrane proteins, the selected marker panel determines which cellular features are evaluated.
Marker results should not be interpreted in isolation. Morphology, differentiation assays, and other biological characteristics add complementary evidence about whether a culture displays the expected MSC properties. This combined assessment is especially relevant when marker profiles are used for quality control or to compare cultures, since surface expression alone does not capture every characteristic of the cells.
Researchers select antibodies directed against the membrane proteins of interest, allow fluorescently labeled antibodies to bind the cells, and then examine the signal using flow cytometry or immunostaining. A useful assessment includes commonly evaluated positive markers, such as CD73, CD90, and CD105, together with negative markers that help identify excluded cell populations.
These measurements are useful when researchers need to isolate or characterize MSC-containing cultures, monitor quality, or compare cells across cultures. The profile can indicate whether expected positive and negative marker patterns are present, while accompanying morphology and differentiation results provide context for interpreting similarities or differences among the cultures.