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Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings
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Neuroscience
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JoVE Journal Neuroscience
Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings

Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings

DOI:
10.3791/53829-v

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08:33 min

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February 26, 2016

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, ,
1Department of Ecology and Evolutionary Biology,University of California, Irvine, 2School of Life Sciences,University of Sussex

Chapters

  • 00:05Title
  • 00:35General Preparation for the Recording
  • 01:13Specimen Preparation and Recording Procedure
  • 07:00Results: Raw Responses and Spectral Sensitivity of Sample Recordings
  • 08:06Conclusion

Summary

Automatic Translation

Automatic Translation

The electrophysiological technique of intracellular recording is demonstrated and used to determine spectral sensitivities of single photoreceptor cells in the compound eye of a butterfly.

Tags

Keywords: Photoreceptor CellSpectral SensitivityIntracellular RecordingsInsect Compound EyeFiltering PigmentsSpectral SensitivitiesMicroelectrodePotassium ChlorideButterflyProboscisAntennaeWingsAbdomenReference ElectrodeCorneaVaselineHead StageAlligator ClipsElectrode HolderPotassium Chloride Solution

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