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A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins

A Miniaturized Glycan Microarray Assay for Assessing Avidity and Specificity of Influenza A Virus Hemagglutinins

DOI:
10.3791/53847-v

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10:32 min

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May 29, 2016

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, ,
1Department of Cell and Molecular Biology, Chemical Physiology and Microbial Science,The Scripps Research Institute, 2Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences,Utrecht University

Chapters

  • 00:05Title
  • 00:42Glycan Preparation and Plate Setup
  • 01:56Printing the Arrays
  • 03:49Humidifying, Numbering, and Blocking the Arrays
  • 05:10Analyzing Glycan Binding Proteins
  • 06:55Slide Scanning and Analysis
  • 08:21Results: Polyacrylamide (PAA) Array Specificity and Discrimination
  • 10:00Conclusion

Summary

Automatic Translation

Automatic Translation

Using a printed glycan microarray strategy, a conventional 96-well plate assay was miniaturized for analysis of influenza A virus hemagglutinin avidity and specificity for sialic acid containing receptors.

Tags

Keywords: Glycan MicroarrayInfluenza A VirusHemagglutininReceptor BindingAviditySpecificityGlycan Sample PreparationPrinting BufferPAA-conjugated GlycansMonovalent GlycansAmine-functionalized Dyes384-well Microtiter PlateArrayerPoly-L-lysine Coated SlidesHumidity Control

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