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Combined Recording of Mechanically Stimulated Afferent Output and Nerve Terminal Labelling in Mouse Hair Follicle Lanceolate Endings
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Combined Recording of Mechanically Stimulated Afferent Output and Nerve Terminal Labelling in Mouse Hair Follicle Lanceolate Endings

Combined Recording of Mechanically Stimulated Afferent Output and Nerve Terminal Labelling in Mouse Hair Follicle Lanceolate Endings

DOI:
10.3791/53854-v

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13:04 min

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May 07, 2016

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, ,
1School of Medicine, Medical Sciences & Nutrition,University of Aberdeen, 2Departments of Pharmacology & Biostatistics,Alfaisal University, 3School of Biological & Biomedical Sciences,University of Durham

Chapters

  • 00:05Title
  • 01:13Preparing Ears for Electrophysiology
  • 04:25Electrophysiology Recording Set Up
  • 08:17Recording Stimulus-evoked Action Potentials
  • 10:13Results: Representative Recording from Hair Follicle Lanceolate Nerve Endings
  • 11:40Conclusion

Summary

Automatic Translation

Automatic Translation

A simple and novel technique for recording afferent discharge due to mechanical stimulation of lanceolate terminals of palisade endings innervating mouse ear skin hair follicles is presented.

Tags

Keywords: Mechanically StimulatedAfferent OutputNerve Terminal LabellingMouse Hair FollicleLanceolate EndingsLive Cell ImagingElectrophysiological RecordingMechanosensationSynaptic-like VesiclesMechanotransducer ChannelsHair Follicle AfferenceMuscle SpindlesMerkel Sensory TerminalsBaroreceptorsMechanical SensorsTrigeminal NerveGreat Auricular NerveExternal Auditory MeatusPinna Skin

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