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Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types
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Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types

Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types

DOI:
10.3791/53916-v

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09:31 min

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May 10, 2016

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, ,
1University of Zürich & Zürich-Basel Plant Science Center

Chapters

  • 00:05Title
  • 00:51Tissue Embedding, Thin Sectioning and Mounting for LAM
  • 04:14LAM
  • 07:32Results: Identification of Genes Expressed Only in the Apomictic Egg Cell
  • 08:33Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol for laser-assisted microdissection of specific plant cell types for transcriptional profiling. While the protocol is suitable for different species and cell types, the focus is on highly inaccessible cells of the female germline important for sexual and apomictic reproduction in the crucifer genus Boechera.

Tags

Keywords: Laser-assisted MicrodissectionLAMTranscriptional ProfilingCell TypesPlant Developmental GeneticsSexual And Asexual ReproductionRare Cell TypesAtherogenesisParaffin WaxMicrotomeGametophyteRNA Extraction

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