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JoVE Journal Bioengineering
Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Article DOI: 10.3791/53942-v • 10:47 min • March 24th, 2016
March 24th, 2016 •
1, 1, 1
1W. M. Keck FTMS Laboratory for Human Health Research, Department of Chemistry, North Carolina State University

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Summary
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A mass spectrometry imaging (MSI) source operated at atmospheric pressure was developed by coupling mid-infrared laser desorption and electrospray post-ionization. Exogenous ice matrix was used as the energy-absorbing matrix to facilitate resonant desorption of tissue-related material. This manuscript provides a step-by-step protocol for performing IR-MALDESI MSI of whole-body neonatal mouse.

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Whole-body Mass Spectrometry Imaging Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI) Spatial Distribution Endogenous Analytes Exogenous Analytes Water Content Variations Atmospheric Pressure Ice Matrix Drug Distribution Analysis Targeted Whole Body Imaging Quantitative Drug Distribution Analysis IR-MALDESI MSI Experiments Optimized Geometry Laser Synchronization Stage Synchronization Mass Spectrometer Synchronization Specimen Preparation OCT Mounting Medium Cryostat Specimen Disc
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