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A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood
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Immunology and Infection
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JoVE Journal Immunology and Infection
A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood

A RAPID Method for Blood Processing to Increase the Yield of Plasma Peptide Levels in Human Blood

DOI:
10.3791/53959-v

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11:36 min

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April 28, 2016

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, , , , , , , , ,
1Charité Center for Internal Medicine and Dermatology, Division General Internal and Psychosomatic Medicine,Charité Universitätsmedizin Berlin, Campus Benjamin Franklin, 2Department of Internal Medicine, Institute of Neurogastroenterology and Motility,Martin-Luther Hospital, Academic Teaching Institution of Charité Universitätsmedizin Berlin, 3Department of Hepatology and Gastroenterology, Molecular Cancer Research Center (MKFZ), Campus Virchow-Klinikum,Charité-Universitätsmedizin Berlin

Chapters

  • 00:05Title
  • 00:59Blood Processing
  • 03:14Recovery of Radiolabled Peptides
  • 05:15High Performance Liquid Chromatography (HPLC) and Radiolabled Ghrelin
  • 06:50Radioimmunoassay
  • 08:42Results: Comparison of RAPID Method Versus Standard Processing for Assessing Plasma Peptide Levels in Human Blood
  • 10:43Conclusion

Summary

Automatic Translation

Automatic Translation

The RAPID blood processing method can be used in humans and yields higher peptide levels as well as allows for assessment of the correct molecular form. Therefore, this method will be a valuable tool in peptide research.

Tags

Keywords: Blood ProcessingRAPID MethodPlasma Peptide LevelsEndogenous Peptide HormonesBlood SamplingBlood CentrifugationRAPID BufferChromatography CartridgesRadioimmunoassayIodine 125 Radiolabeled Peptides

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