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整个山原位对腹足软体动物杂交方法静水椎实螺</em

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Biology

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JoVE Journal Biology

A Whole Mount In Situ Hybridization Method for the Gastropod Mollusc Lymnaea stagnalis

Please note that all translations are automatically generated. Click here for the English version.

整个山原位对腹足软体动物杂交方法静水椎实螺</em

DOI:

10.3791/53968-v

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07:33 min

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March 15, 2016

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Daniel J. Jackson, Ines Herlitze, Jennifer Hohagen

¹Department of Geobiology,Georg-August University of Göttingen

Chapters

00:05Title
00:52Assembly of Decapsulation Apparatus
01:43Sample Collection, Fixation and Decapsulation
05:48Results: Representative Images of WMISH Expression Patterns Against a Variety of Genes from a Range of L. stagnalis Developmental Stages
06:42Conclusion

Summary

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The goal of this protocol is to provide users with a set of methods for the high-throughput decapsulation of Lymnaea stagnalis embryos and larvae in preparation for whole mount in situ hybridization, and for subsequent pre- and post-hybridization treatments.

整个山<em>原位</em>对腹足软体动物杂交方法<em>静水椎实螺</em

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