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Biology

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Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae
 

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Article doi: 10.3791/53975
April 18th, 2016

Summary April 18th, 2016

Protein abundance reflects the rates of both protein synthesis and protein degradation. This article describes the use of cycloheximide chase followed by western blotting to analyze protein degradation in the model unicellular eukaryote, Saccharomyces cerevisiae (budding yeast).

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