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Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
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1New Jersey Center for Science, Technology and Mathematics,Kean University, 2Department of Physiology and Medical Physics,Royal College of Surgeons in Ireland, 3The Feinstein Institute for Medical Research at North Shore-LIJ, 4Department of Anatomy and Structural Biology, Gruss Lipper Biophotonics Center,Albert Einstein College of Medicine
Chapters
- 00:05Title
- 00:44Pre-coated Matrix Chamber Coculture Invasion Assay
- 04:07Invasion Assay: “3D” Embedding of Glioma and Macrophages or Microglia in Matrix
- 06:14Results: Microglia and Macrophage Enhancement of Glioblastoma Cell Invasion
- 08:42Conclusion
Understanding the malignant behavior of cancer requires creating accurate models of how tumor cells interact with components of the tumor microenvironment, such as macrophages. Here we describe two methods to study glioblastoma cell interaction with tumor associated macrophages and microglia where the effect on glioblastoma invasion is assessed.
