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In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila
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In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

In Vivo Biosensor Tracks Non-apoptotic Caspase Activity in Drosophila

DOI:
10.3791/53992-v

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13:21 min

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November 27, 2016

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, , ,
1W. Harry Feinstone Department of Molecular Microbiology and Immunology,Johns Hopkins University Bloomberg School of Public Health, 2School of Life Sciences,Chinese University of Hong Kong

Chapters

  • 00:05Title
  • 01:48Preparing the CaspaseTracker Flies and Dissected Tissues
  • 04:30Tissue Staining and Mounting
  • 08:11Confocal Microscopy
  • 09:37Results: Caspase Biosensor Activity in Non-dying Cells of Healthy Flies
  • 12:10Conclusion

Summary

Automatic Translation

Automatic Translation

To detect healthy cells in whole animals that contain low levels of caspase activity, the highly sensitive biosensor designated CaspaseTracker was generated for Drosophila. Caspase-dependent biosensor activity is detected in long-lived healthy cells throughout the internal organs of adult animals reared under optimized conditions in the absence of death stimuli.

Tags

Keywords: Caspase ActivityIn Vivo BiosensorDrosophila MelanogasterNon-apoptotic CaspaseCaspase TrackerG-TraceDQVDGal4RFPFLP RecombinaseGFPCaspase Cleavage Site

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