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Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
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JoVE Journal Biology
Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix

DOI:
10.3791/54005-v

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10:21 min

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June 14, 2016

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, , , , , ,
1Department of Pathology, Microbiology, and Immunology,Vanderbilt University Medical Center, 2Department of Medicine,Vanderbilt University Medical Center, 3Division of Cardiovascular Medicine,Vanderbilt University Medical Center, 4Cardiovascular Institute,Maine Medical Center

Chapters

  • 00:05Title
  • 01:15Decellularization of Heart Tissue Specimen
  • 02:20Osmication, Dehydration and Cross-sectioning for SEM
  • 04:32Critical Point Drying (CPD) and Mounting for SEM
  • 06:33SEM Examination of Heart Tissue Specimens
  • 08:18Results: Structural and Quantitative Analyses
  • 09:22Conclusion

Summary

Automatic Translation

Automatic Translation

Shown here is a method for visualizing extracellular matrix ultrastructure in decellularized cardiac tissues.

Tags

Keywords: Scanning Electron MicroscopyExtracellular MatrixFibrosisCardiomyopathySodium HydroxideTannic AcidSodium CacodylateOsmium Tetroxide

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