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JoVE Journal Developmental Biology
In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas
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In Vitro Colony Assays for Characterizing Tri-potent Progenitor Cells Isolated from the Adult Murine Pancreas

Article DOI: 10.3791/54016-v • 09:31 min • June 10th, 2016
June 10th, 2016 •
*1,2, *1, 1, 1, 1, 1, 1, 3, 1
1Diabetes and Metabolism Research Institute, Beckman Research Institute of City of Hope, 2Irell & Manella Graduate School of Biological Sciences, Beckman Research Institute of City of Hope, 3Division of Chemistry and Chemical Engineering, California Institute of Technology
* These authors contributed equally

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Summary
Automatic Translation

In vitro colony assays to detect self-renewal and differentiation of progenitor cells isolated from adult murine pancreas are devised. In these assays, pancreatic progenitors give rise to cell colonies in 3-dimensional space in methylcellulose-containing semi-solid medium. Protocols for handling single cells and characterization of individual colonies are described.

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In Vitro Colony Assays Characterizing Tri-potent Progenitor Cells Adult Murine Pancreas Protocols Pancreatic Progenitor Cells Methylcellulose-containing Semi-solid Medium Self-renewal Differentiation Capacities Individual Progenitor Cells Biological Potentials Hematopoietic Stem Cells Pancreatic Cell Populations Jacob Tremblay Janine Quijano Angela Luo Laboratory Vertical Incision Abdominal Wall Abdominal Cavity Spleen Connective Tissue Splenic Lobe Duodenal Lobe Gastric Lobe Petri Dish DPBS BSA Antibiotics
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