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Ekspression og oprensning af viruslignende partikler til vaccination
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JoVE Journal Biology
Expression and Purification of Virus-like Particles for Vaccination

Ekspression og oprensning af viruslignende partikler til vaccination

DOI:
10.3791/54041-v

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06:17 min

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June 02, 2016

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, ,
1Center for Vaccines and Immunology, Department of Infectious Diseases, College of Veterinary Medicine,University of Georgia

Chapters

  • 00:05Title
  • 00:58Influenza H3N2 Virus-like Particle (VLP) Generation
  • 02:10CHIK VLP Expression Using Baculovirus/Insect Cell System
  • 03:18Sedimentation and Purification of VLPs and SVPs
  • 04:42Results: Successful Expression and Purification of VLPs
  • 05:23Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi en protokol til at syntetisere viruslignende partikler ved hjælp af enten baculovirus eller pattedyrekspressionssystemer og ultracentrifugering rensning. Denne meget tilpasselig tilgang bruges til at identificere virale antigener som vaccine mål på en sikker og fleksibel måde.

Tags

Virus-like ParticlesVLPsVaccinationProtein ExpressionPurificationMammalian CellsInsect CellsTransfectionBaculovirusCHIKVAntigenVaccineSerologyDiagnosis

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