JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
表达和疫苗病毒样颗粒的纯化
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Expression and Purification of Virus-like Particles for Vaccination
Please note that all translations are automatically generated.
Click here for the English version.
表达和疫苗病毒样颗粒的纯化
DOI:
10.3791/54041-v
•
06:17 min
•
June 02, 2016
•
Maria T. Arevalo
,
Terianne M. Wong
,
Ted M. Ross
1
Center for Vaccines and Immunology, Department of Infectious Diseases, College of Veterinary Medicine
,
University of Georgia
Chapters
00:05
Title
00:58
Influenza H3N2 Virus-like Particle (VLP) Generation
02:10
CHIK VLP Expression Using Baculovirus/Insect Cell System
03:18
Sedimentation and Purification of VLPs and SVPs
04:42
Results: Successful Expression and Purification of VLPs
05:23
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
这里,我们提出用于合成使用任一杆状病毒或哺乳动物表达系统,并超速离心纯化病毒样颗粒的协议。这种高度可定制的方式被用来识别在一个安全的,灵活的方式的病毒抗原作为疫苗的目标。
Tags
Virus-like Particles
VLPs
Vaccination
Protein Expression
Purification
Mammalian Cells
Insect Cells
Transfection
Baculovirus
CHIKV
Antigen
Vaccine
Serology
Diagnosis
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
主电穿孔的小鼠骨髓造血细胞培养制备
无有机溶剂和醋酸,蛋白质与考马斯亮蓝G - 250凝胶染色
使用基因脉冲MXcell电穿孔系统主细胞的转染效率高
使用自动细胞计数器简化基因表达研究:在Namalwa细胞的IL - 4依赖的基因表达的siRNA击倒
表达,洗涤剂溶解,和一个膜转运,MexB多药耐药蛋白的纯化
石蜡包埋及冷冻的部分
果蝇
成人肌肉
在体外
重组活跃
T。谷盗
端粒酶
囊性纤维化跨膜传导调节蛋白表达和纯化
酿酒酵母
成绩单和代谢产物的分离纯化<em>果蝇</em>头
全球合成的RNA在单细胞水平以下的低氧分析
Read Article