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Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly
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JoVE Journal Biology
Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly

DOI:
10.3791/54064-v

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09:51 min

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April 25, 2016

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, ,
1Department of Biology,Ouachita Baptist University

Chapters

  • 00:05Title
  • 01:28Annealing Oligonucleotides (Applies Only to Freeze-dried DNA)
  • 02:43Golden Gate Assembly (Cost Efficient Method Using a Standard Mix)
  • 04:19Transformation and Colony Selection
  • 05:19Verification and Quantification of Terminator
  • 07:55Quantification of Six Different Terminators in pGR-Blue
  • 09:02Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol utilizes Golden Gate Assembly and the plasmid pGR-blue to rapidly quantify the strength of terminators found in silico.

Tags

TerminatorIn-vitroGolden Gate AssemblyBio-informaticsRapid VerificationPGR-Blue PlasmidOligonucleotidesAnnealingRestriction EnzymeLigaseCloning Efficiency

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