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An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons
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JoVE Journal Neuroscience
An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons

An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons

DOI:
10.3791/54085-v

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09:48 min

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August 24, 2016

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,
1Department of Chemical Engineering and Materials Science,Michigan State University, 2Department of Biochemistry and Molecular Biology,Michigan State University

Chapters

  • 00:05Title
  • 00:41Preparation of Pre-stretched Anisotropic Surface
  • 02:22Isolation of DRG
  • 05:07Culture of DRG on Pre-stretched Surface
  • 06:12Co-culture of Schwann Cells with DRG Neurons on Pre-stretched Surface
  • 07:54Results: Co-cultured Schwann Cells with Aligned DRG Axons on Stretched vs. Unstretched PDMS Substrates
  • 08:42Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the isolation of dorsal root ganglion (DRG) neurons isolated from rats and the culture of DRG neurons on a static pre-stretched cell culture system to enhance axon alignment, with subsequent co-culture of Schwann Cells (SCs) to promote myelination.

Tags

Keywords: Dorsal Root Ganglion NeuronsAxon AlignmentMyelinationPre-stretched Cell Culture SystemPDMS MembraneOxygen Plasma TreatmentPLLDRG Neuron IsolationSD Rats

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