Hybridization Capture
Hybridization Capture - Related Videos
09:27
Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells
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Cited by 2 •
2018
This is a method to identify novel DNA-interacting proteins at specific target loci, relying on sequence-specific capture of crosslinked chromatin for subsequent proteomic analyses. No prior knowledge about potential binding proteins, nor cell modifications are required. Initially developed for yeast, the technology has now been adapted for mammalian cells.
03:24
Hybridization of Atomic Orbitals I
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2020
The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...
08:00
Whole-Mount In Situ Hybridization
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2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
03:35
Hybridization of Atomic Orbitals II
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2020
sp3d and sp3d 2 Hybridization To describe the five bonding orbitals in a trigonal bipyramidal arrangement, we must use five of the valence shell atomic orbitals (the s orbital, the three p orbitals, and one of the d orbitals), which gives five sp3d hybrid orbitals. With an octahedral arrangement of six hybrid orbitals, we must use six valence shell atomic orbitals (the s orbital, the three p orbitals, and two of the d orbitals in its valence shell), which gives six sp3d 2 hybrid orbitals.
02:14
Prey Capture Assay: A Method to Study the Prey Capture Behavior of Zebrafish Larva
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2023
This video describes the prey capture assay. The video discusses the method to study prey capture behavior of zebrafish larvae after the laser ablation of specific neurons.
FAQs
Complementary base pairing gives the method its molecular selectivity. A probe binds a target sequence to form a probe-target duplex, while unrelated molecules remain unpaired under the chosen hybridization conditions. This distinction allows the target to be concentrated from a complex sample before downstream analysis, rather than treating every molecule equally.
Controlled hybridization conditions determine whether complementary probe and target sequences form stable duplexes. If pairing is not sufficiently favored, target enrichment can be reduced; if the conditions do not distinguish the intended pairing from unrelated molecules, selectivity may suffer. Thus, the conditions directly influence the quality and usefulness of the recovered material.
The label provides a way to distinguish probe-associated material from unbound sample molecules after hybridization. Affinity-based separation then retains the labeled probe-target complexes while unbound molecules are removed. Recovery of the retained material produces an enriched fraction suitable for subsequent genetic analysis, concentrating sequences that might otherwise remain difficult to detect.
Researchers first combine the complex biological sample with labeled probes under controlled conditions, allowing complementary targets to form duplexes. They then separate probe-associated material from unbound molecules, remove the latter, and recover the captured fraction. That sequence converts a mixed sample into material enriched for selected nucleic acid regions for further analysis.
The technique is valuable when informative genetic material is scarce, low abundance, degraded, or restricted to selected regions. Targeted sequencing and pathogen detection can focus analysis on material of interest, while genomic variation studies can examine chosen sequences without analyzing an entire genome. This focused strategy can also reduce sequencing costs compared with whole-genome analysis.
It concentrates selected regions before sequencing or other analysis, allowing researchers to prioritize informative genetic material rather than distribute effort across the full genome. In biology, this focus supports genomic variation analysis, pathogen detection, and studies of degraded DNA. Enrichment can improve detection sensitivity while reducing the amount of sequencing devoted to unrelated regions.