Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Geetika Sahni; Jun Yuan; Yi-Chin Toh

doi:10.3791/54097

Journal

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Developmental Biology

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Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

8,492 Views

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08:07 min

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June 17, 2016

DOI:

10.3791/54097-v

DOI:

10.3791/54097-v

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08:07 min

June 17, 2016

•

8466 Views

Geetika Sahni*¹, Jun Yuan*¹, Yi-Chin Toh^1,2

¹Department of Biomedical Engineering,National University of Singapore ²Singapore Institute of Neurotechnology (SINAPSE),National University of Singapore

Summary

Human pluripotent stem cells (hPSCs) have the intrinsic ability to differentiate and self-organize into distinct tissue patterns; although this requires the presentation of spatial environmental gradients. We present stencil micropatterning as a simple and robust method to generate biochemical and mechanical gradients for controlling hPSC differentiation patterns.