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JoVE Journal Developmental Biology
Stencil Micropatterning for Human pluripotente stamceller til Sondering Spatial Organisation af differentiering Fates
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Stencil Micropatterning for Human pluripotente stamceller til Sondering Spatial Organisation af differentiering Fates

Article DOI: 10.3791/54097-v • 08:07 min • June 17th, 2016
June 17th, 2016 •
*1, *1, 1,2
1Department of Biomedical Engineering, National University of Singapore, 2Singapore Institute of Neurotechnology (SINAPSE), National University of Singapore
* These authors contributed equally

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Humane pluripotente stamceller (hPSCs) har den iboende evne til at differentiere og selv-organisere sig i forskellige væv mønstre; selvom dette kræver præsentation af rumlige miljømæssige gradienter. Vi præsenterer stencil micropatterning som en enkel og robust metode til at generere biokemiske og mekaniske gradienter til styring hPSC differentieringsmønstre.

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Developmental Biology Humane pluripotente stamceller celle micropatterning stencil differentiering skæbne fysisk planlægning væv mønster fosterudvikling
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