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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

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JoVE Journal Biology

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

DOI:

10.3791/54121-v

•

07:44 min

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July 06, 2016

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Stefanie Arndt*¹, Heidi-Kristin Walter*¹, Hans-Achim Wagenknecht

¹Institute of Organic Chemistry,Karlsruhe Institute of Technology

Chapters

00:05Title
00:40Synthesis of the Dyes
01:48Synthesis of the DNA Strands
02:25“Clicking” Procedure and HPLC Purification
04:15Sample Preparation and Spectroscopy
05:52Results: Absorption and Fluorescence Spectroscopy Changes upon DNA Hybridization
07:18Conclusion

Summary

Automatic Translation

Photostable cyanine dyes are attached to oligonucleotides to monitor hybridization by energy transfer.

Tags

Keywords: DNA Hybridization Wavelength-shifting Probes Cyanine Dyes Fluorescence RNA Interference SiRNA DNA Synthesis DNA Purification HPLC

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