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JoVE Journal
Developmental Biology

This content is Open Access.

Isolation of Murine Embryonic Hemogenic Endothelial Cells
 

Isolation of Murine Embryonic Hemogenic Endothelial Cells

Article doi: 10.3791/54150
June 17th, 2016

Summary June 17th, 2016

Hematopoietic stem and progenitor cells (HSPC) derive from specialized (hemogenic) endothelial cells during development, yet little is known about the process by which some endothelial cells specify to become blood forming. We demonstrate a flow-cytometry based method allowing simultaneous isolation of hemogenic endothelial cells and HSPC from murine embryonic tissues.

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