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Journal / Developmental Biology / Isolatie van muizen embryonale Hemogenic…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolatie van muizen embryonale Hemogenic endotheelcellen
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Isolatie van muizen embryonale Hemogenic endotheelcellen

Article DOI: 10.3791/54150-v • 08:56 min • June 17th, 2016
June 17th, 2016 •
*1, *2, 3, 1
1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center, Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine, Yale University School of Medicine, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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Hematopoietische stamcellen en progenitorcellen (HSPC) afgeleid van gespecialiseerde (hemogenic) endotheelcellen tijdens de ontwikkeling, maar weinig bekend over het proces waarbij sommige endotheelcellen specificeren bloedvormende worden. We tonen een stroom-cytometrie-gebaseerde methode waarmee gelijktijdige isolatie van hemogenic endotheelcellen en HSPC van muizen embryonale weefsels.

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Developmental Biology hemogenic endotheel definitieve hematopoiese vasculaire ontwikkeling muis embryo's FACS hematopoietische stamcellen en stamcellen
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