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JoVE Journal
Biology

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Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells
 

Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells

Article DOI: 10.3791/54176 09:12 min July 22nd, 2016
July 22nd, 2016

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Summary

Primary human bronchial epithelial cells are difficult to transduce. This protocol describes the production of lentiviruses and their concentration as well as the optimal culture conditions necessary to achieve highly efficient transductions in these cells throughout differentiation to a pseudostratified epithelium.

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Lentivirus Production Cell Culture Conditions Transduction Efficiency Primary Human Bronchial Epithelial Cells Lentiviruses BSL-2 Conditions HEK Cells Transfection Kit PMD2-VSVG Envelope DNA PMDLg/pRRE Packaging DNA PRSV-rev DNA Lentiviral Expression Vector Calcium Solution
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